The Symbolism of Hester Prynnes Appearance in The Scarlet Letter :: Essays Papers

The Symbolism of Hester Prynnes Appearance in The Scarlet Letter Throughout The Scarlet Letter, the author Nathaniel Hawthorne uses many literal and figurative items to illustrate the significance of various characters or themes. Coupled with the tangible evidence given, the reader can make many miscellaneous assumptions of the importance of these items to directly and indirectly contribute to the issues of the novel. One cryptic item that symbolizes more than it seems to is the appearance of one of the main characters, Hester Prynne. Hester Prynne's appearance and hair signify the levels of will and determination she possesses at the moment. The first example of this implication occurs at the beginning of the novel, during and before the first scaffold scene. Hester's hair is let down, shining and "so glossy that it threw off the sunshine with a gleam." This hair compliments an elegant, beautiful woman standing tall and proud. During this time, Hester's emotions, at full integrity, mirroring her appearance. When Hester is being questioned upon the scaffold, her virtue shines through when she refuses to name the partner of her sin. In the next example, Hester's pride and stature both seem to dwindle in accordance to her appearance. Within the next seven years, Hester has gone through a change both physically and emotionally. The book describes the scarlet letter to have absorbed all the rebellious and fiery qualities of Hester, leaving a cold and lonely woman, her tenderness "crushed so deeply into her heart that it can never show itself more." At the same time, Hester started "hiding" her beautiful rich hair in a cap, therefore practically eliminating her beauty and femininity. As Hester becomes less passionate internally, she becomes less passionate externally as well. The last example takes place during Hester's talk with Arthur Dimmesdale in the forest. During their emotional conversation, Hester regains her previous composure, and tries to prove to Arthur that the past can be erased. With the literal simple gestures of pulling off her scarlet letter and taking off her cap, she figuratively obliterated all that had happened before she was forced to wear the scarlet letter. Her womanliness and pride came rushing back as her hair fell back to her shoulders, becoming as she was before the scarlet letter took over her life. The Symbolism of Hester Prynnes Appearance in The Scarlet Letter :: Essays Papers The Symbolism of Hester Prynnes Appearance in The Scarlet Letter Throughout The Scarlet Letter, the author Nathaniel Hawthorne uses many literal and figurative items to illustrate the significance of various characters or themes. Coupled with the tangible evidence given, the reader can make many miscellaneous assumptions of the importance of these items to directly and indirectly contribute to the issues of the novel. One cryptic item that symbolizes more than it seems to is the appearance of one of the main characters, Hester Prynne. Hester Prynne's appearance and hair signify the levels of will and determination she possesses at the moment. The first example of this implication occurs at the beginning of the novel, during and before the first scaffold scene. Hester's hair is let down, shining and "so glossy that it threw off the sunshine with a gleam." This hair compliments an elegant, beautiful woman standing tall and proud. During this time, Hester's emotions, at full integrity, mirroring her appearance. When Hester is being questioned upon the scaffold, her virtue shines through when she refuses to name the partner of her sin. In the next example, Hester's pride and stature both seem to dwindle in accordance to her appearance. Within the next seven years, Hester has gone through a change both physically and emotionally. The book describes the scarlet letter to have absorbed all the rebellious and fiery qualities of Hester, leaving a cold and lonely woman, her tenderness "crushed so deeply into her heart that it can never show itself more." At the same time, Hester started "hiding" her beautiful rich hair in a cap, therefore practically eliminating her beauty and femininity. As Hester becomes less passionate internally, she becomes less passionate externally as well. The last example takes place during Hester's talk with Arthur Dimmesdale in the forest. During their emotional conversation, Hester regains her previous composure, and tries to prove to Arthur that the past can be erased. With the literal simple gestures of pulling off her scarlet letter and taking off her cap, she figuratively obliterated all that had happened before she was forced to wear the scarlet letter. Her womanliness and pride came rushing back as her hair fell back to her shoulders, becoming as she was before the scarlet letter took over her life.

Plant Tissue Culture Essay

Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues or organs under sterile conditions on a nutrient culture medium of known composition. Plant tissue culture is widely used to produce clones of a plant in a method known as micropropagation. Different techniques in plant tissue culture may offer certain advantages over traditional methods of propagation, including: †¢The production of exact copies of plants that produce particularly good flowers, fruits, or have other desirable traits. †¢To quickly produce mature plants. †¢The production of multiples of plants in the absence of seeds or necessary pollinators to produce seeds. †¢The regeneration of whole plants from plant cells that have been genetically modified. †¢The production of plants in sterile containers that allows them to be moved with greatly reduced chances of transmitting diseases, pests, and pathogens. †¢The production of plants from seeds that otherwise have very low chances of germinating and growing, i.e.: orchids and nepenthes. †¢To clean particular plants of viral and other infections and to quickly multiply these plants as ‘cleaned stock’ for horticulture and agriculture. Plant tissue culture relies on the fact that many plant cells have the ability to regenerate a whole plant (totipotency). Single cells, plant cells without cell walls (protoplasts), pieces of leaves, or (less commonly) roots can often be used to generate a new plant on culture media given the required nutrients and plant hormo nes. Techniques Modern plant tissue culture is performed under aseptic conditions under HEPA filtered air provided by a laminar flow cabinet. Living plant materials from the environment are naturally contaminated on their surfaces (and sometimes interiors) with microorganisms, so surface sterilization of starting material (explants) in chemical solutions (usually sodium or calcium hypochlorite or mercuric chloride) is required. Mercuric chloride is seldom used as a plant sterilant today, unless other sterilizing agents are found to be ineffective, as it is dangerous to use, and is difficult to dispose of. Explants are then usually placed on the surface of a solid culture medium, but are sometimes placed directly into a liquid medium, particularly when cell suspension cultures are desired. Solid and liquid media are generally composed of inorganic salts plus a few organic nutrients, vitamins and plant hormones. Solid media are prepared from liquid media with the addition of a gelling agent, usually p urified agar. In vitro tissue culture potato explants The composition of the medium, particularly the plant hormones and the nitrogen source (nitrate versus ammonium salts or amino acids) have profound effects on the morphology of the tissues that grow from the initial explant. For example, an excess of auxin will often result in a proliferation of roots, while an excess of cytokinin may yield shoots. A balance of both auxin and cytokinin will often produce an unorganised growth of cells, or callus, but the morphology of the outgrowth will depend on the plant species as well as the medium composition. As cultures grow, pieces are typically sliced off and transferred to new media (subcultured) to allow for growth or to alter the morphology of the culture. The skill and experience of the tissue culturist are important in judging which pieces to culture and which to discard. As shoots emerge from a culture, they may be sliced off and rooted with auxin to produce plantlets which, when mature, can be transferred to potting soil for further g rowth in the greenhouse as normal plants. [1] Choice of explant The tissue obtained from the plant to culture is called an explant. Based on work with certain model systems, particularly tobacco, it has often been claimed that a totipotent explant can be grown from any part of the plant. However, this concept has been vitiated in practice. In many species explants of various organs vary in their rates of growth and regeneration, while some do not grow at all. The choice of explant material also determines if the plantlets developed via tissue culture are haploid or diploid. Also the risk of microbial contamination is increased with inappropriate explants. Thus it is very important that an appropriate choice of explant be made prior to tissue culture. The specific differences in the regeneration potential of different organs and explants have various explanations. The significant factors include differences in the stage of the cells in the cell cycle, the availability of or ability to transport endogenous growth regulators, and the metabolic capabilities of the cells. The most commonly used tissue explants are the meristematic ends of the plants like the stem tip, auxiliary bud tip and root tip. These tissues have high rates of cell division and either concentrate or produce required growth regulating substances including auxins and cytokinins. The pathways through which whole plants are regenerated from cells and tissues or explants such as meristems broadly fall into three types: 1.The method in which explants that include a meristem (viz. the shoot tips or nodes) are grown on appropriate media supplemented with plant growth regulators to induce proliferation of multiple shoots, followed by rooting of the excised shoots to regenerate whole plants, 2.The method in which totipotency of cells is realized in the form of de novo organogenesis, either directly in the form of induction of shoot meristems on the explants or indirectly via a callus (unorganised mass of cells resulting from proliferation of cells of the explant) and plants are regenerated through induction of roots on the resultant shoots, 3.Somatic embryogenesis, in which asexual adventive embryos (comparable to zygotic embryos in their structure and development) are induced directly on explants or indirectly through a callus phase. The first method involving the meristems and induction of multiple shoots is the preferred method for the micropropagation industry since the risks of somaclonal variation (genetic variation induced in tissue culture) are minimal when compared to the other two methods. Somatic embryogenesis is a method that has the potential to be several times higher in multiplication rates and is amenable to handling in liquid culture systems like bioreac tors. Some explants, like the root tip, are hard to isolate and are contaminated with soil microflora that become problematic during the tissue culture process. Certain soil microflora can form tight associations with the root systems, or even grow within the root. Soil particles bound to roots are difficult to remove without injury to the roots that then allows microbial attack. These associated microflora will generally overgrow the tissue culture medium before there is significant growth of plant tissue. Aerial (above soil) explants are also rich in undesirable microflora. However, they are more easily removed from the explant by gentle rinsing, and the remainder usually can be killed by surface sterilization. Most of the surface microflora do not form tight associations with the plant tissue. Such associations can usually be found by visual inspection as a mosaic, de-colorization or localized necrosis on the surface of the explant. An alternative for obtaining uncontaminated explants is to take explants from seedlings which are aseptically grown from surface-sterilized seeds. The hard surface of the seed is less permeable to penetration of harsh surface sterilizing agents, such as hypochlorite, so the acceptable conditions of sterilization used for seeds can be much more stringent than for vegetative tissues. Tissue cultured plants are clones. If the original mother plant used to produce the first explants is susceptible to a pathogen or environmental condition, the entire crop would be susceptible to the same problem. Conversely, any positive traits would remain within the line also. Applications Plant tissue culture is used widely in plant science; it also has a number of commercial applications. Applications include: †¢Micropropagation is widely used in forestry and in floriculture. Micropropagation can also be used to conserve rare or endangered plant species.[2] †¢A plant breeder may use tissue culture to screen cells rather than plants for advantageous characters, e.g. herbicide resistance/tolerance. †¢Large-scale growth of plant cells in liquid culture in bioreactors for production of valuable compounds, like plant-derived secondary metabolites and recombinant proteins used as biopharmaceuticals.[3] †¢To cross distantly related species by protoplast fusion and regeneration of the novel hybrid. †¢To cross-pollinate distantly related species and then tissue culture the resulting embryo which would otherwise normally die (Embryo Rescue). †¢For production of doubled monoploid (dihaploid) plants from haploid cultures to achieve homozygous lines more rapidly in breeding programmes, usually by treatment with colchicine which causes doubling of the chromosome number. †¢As a tissue for transformation, followed by either short-term testing of genetic constructs or regeneration of transgenic plants. †¢Certain techniques such as meristem tip culture can be used to produce clean plant material from virused stock, such as potatoes and many species of soft fruit. †¢Micropropagation using meristem and shoot culture to produce large numbers of identical individuals. †¢Production of identical sterile hybrid species can be obtained. Laboratories Although some growers and nurseries have their own labs for propagating plants by the technique of tissue culture, a number of independent laboratories provide custom propagation services. The Plant Tissue Culture Information Exchange lists many commercial tissue culture labs. Since plant tissue culture is a very labour intensive process, this would be an important factor in determining which plants would be commercially viable to propagate in a laboratory.

Resources And Time Management - Free Essay Example

Sample details Pages: 3 Words: 1016 Downloads: 10 Date added: 2019/05/29 Category Management Essay Level High school Tags: Time Management Essay Did you like this example? Resource and Time Management Resources and time management play an important role in making a project success. Organizations rely on resources to carry out the project tasks. Time management is very critical in the project completion. The resources need to be able to follow the process and make sure to finish the tasks on time. Without the correct team/resources set up, any procedure and plan have the capability of totally falling apart. In my opinion, project manager and entire team needs to work in a collaborative style, that is really important to ensure the success of a project and to finish the project on time. According to Kerzner (2001), project success was clearly explained as the completion of a project within the specified budget, time, performance. This de? ¬nition of project success has been modified to incorporate completion within the mentioned time period, at the possible execution or specification level. Don’t waste time! Our writers will create an original "Resources And Time Management" essay for you Create order A project can be viewed as effective by meeting the execution measures of cost, time and technical performance, but also ensuring and safeguarding that the project is acknowledged and accepted by the client (Kerzner 1998). I would like to concentrate more on time, resource performance and of course customer satisfaction. Success will be determined by the level of execution accomplished. To decide whether these factors are actually useful and success will be depending on their impact on project execution. Belassi and Tukel (1996) grouped critical success factors into four zones: external environment, team, organization and the project. The identi? ¬cation of critical factors would prompt the better assessment of projects. Pinto and Slevin (1987) found ten critical success factors, including project mission, top administration support, project schedule/plan, customer consultation, personnel, technical tasks, client acceptance, monitoring and feedback, communication and investigating. This exploration just identified the critical success factors, yet did not quantify the quality of their association with project performance. Cooke-Davies (2002) recommended a range of critical success factors related to performance, including project team and management competencies relevant to huge, complex projects, but also to projects in general. Project management incorporates productively overseeing individuals and partnerships viably. The decision power should be given to sponsor and the sponsor must work with project manager and they need to be in a decent understanding about the wants of a project. The project manager needs to be clear on making the requirements clear with the management. And the leadership should really give the decision power to the sponsor who will then take care on getting requirements done quickly. We need to be clear and transparent with the stakeholders that help them understand the performance. Communicate with upper management and come to an understanding on the decisions and have their support financially. The sponsor needs to be available and help in making decisions and influencing the stakeholders and management. Team work needs communication, coordination ability to do exploration on the tasks. Make sure involve right person on right time. Include the stakeholders in meetings in order to better identify if any issues at that moment. As work demands increase, managers need to manage time and work projects effectively. Knowing how you invest your time is the initial step to enhance and improve efficiency and time management. Managers ought to distinguish performance time and wasteful time utilize. MANAGING TIME Every team member should manage their time effectively and work to achieve project success, some of the basic things to consider: Try to work on the critical stuff on priority and schedule the less important or priority tasks to later. Also, make sure you dont switch tasks, that leads to time loss understanding both. Raffoni (2006) reports that switching between tasks could diminish effectiveness by 20% to 40%. Since email interferences can distract from work and take time to read and reply to e-mails. Enhancing time and project management incudes creating work time without interferences. When working on a project, do not end up interrupted by unnecessary talk from colleagues. The tasks need to show the manageable steps of the project. Also, they ought to be divided into subtasks which take less time to complete. It is ok to say no to colleagues when they try to give you more work or ask questions when you do not have time. Organization is another key factor in project and time management for managers. Workplace organization is critical to work effectively. Time spent searching for files, folders, or projects is considered waste. Keep moving towards technology which gives us the features to do our work in less time. Because many files are now electronic, retain only essential hard copy documents. Often update and back up the files that you complete. Eliminate duplicates and rough drafts of completed work. This greatly reduces manual work and saves time. All the team members ought to be communicating within themselves often and offer the ideas. Following agile really helps in determining the tasks and also the fixes if something goes wrong. Agile is flexible and the team members and project manager should follow the principles of agile methodology. Utilize the tools available to you to reduce work load and make sure everything is done on time. Would like to discuss on more on agile methodologies which the team uses, it involves communication like handling stand-up calls and the progress made everyday by team members. Having code reviews by the team and automate the tasks to test. Follow agile principles and methodologies in project, more focus to individuals than to process and tools. Just make sure to provide adequate documentation. Respond to changes quickly. Give more importance to customer feedback than the requirements defined. Of course we need to follow requirements and develop the tasks but when comes to customer satisfaction and needs, we need to give preference to the client. Keeping to the timetable in which the design and requirements are created. And to how well the team can convey the outcomes characterized in scope. We often hear organizations have been faced with problems in delivering, so the project manager should make sure we dont go beyond scope and timelines. Then the team can work towards increasing speed and in post-execution adjustments accordingly.